Pathogen identification
The disease can be detected based on the presence of fruiting bodies or basidiomata or sporophore of the Ganoderma on the palm bole or roots.
Isolation of Ganoderma
The diseased tissue is directly placed onto the medium without the need for surface sterilisation. A brown halo around the colony indicates the presence of Ganoderma on the GSM.
Collecting field samples of infected stem/trunk, roots or fruiting body of Ganoderma
Plating samples onto the GSM and incubation at 28ºC for 5-8 days. With GSM, washing and sterilisation of the diseased tissues become unnecessary and transfer of the samples could also be done in the field
The presence of Ganoderma is confirmed with a brown halo. The white mycelium is then transferred to potato dextrose agar (PDA)
Pure culture of Ganoderma fungus on PDA after identifying the morphological characteristics using microscope
